|
KMID : 0352519860230030115
|
|
Korea Univercity Medical Journal
1986 Volume.23 No. 3 p.115 ~ p.121
|
|
|
Formation of neutralizing antibodies against Hantaan and Seoul virus of sera from hemorrhagic fever with renal syndrome patients in Korea
|
|
|
|
|
Abstract
|
|
|
The production of neutralizing antibodies against Hantaan and Seoul viruses in sera of five patients diagnosed as typical Korean hemorrhagic fever were investigated using plaque reduction neutralization test. Cross reactivity of sera of the patients to Hantaan and Seoul viruses which are related but distinct antigenically were also studied.
The patients with Korean hemorrhagic fever have been bled by weekly intervals from 3 days to 66 days after the onset of illness and sera were prepared and stored in -65C until use.
Neutralizing antibody titers against Hantaan and Seoul viruses were determined by a serum dilution-plaque reduction neutralization (PRN) method employing immunoperoxidase staining.
In gerneral, the antibodies were detected from as early as 3 days after the onset of disease. The peak titers were reached at around 2 weeks after the onset of illness and the antibodies were persisted up to 66 days although the titers were decreased slowly.
All sera used were neutralized Hantaan viruses in higher titers in contrast with lower titers with Seoul viruses.
It can be concluded that HFRS patients in this study were infected with Hantaan virus in rural areas of Korea.
|
|
|
KEYWORD
|
|
|
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|
|